ZFIN Image: Ge et al., 2022, Figure 2

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Figure 2

The workflow of RNA−seq data analysis. (A) Main steps of RNA−seq data analysis in the study. (B) Statistics for mapping of reads in four groups. The sample names used the experimental temperatures. Temperatures at 19.5 °C and 19 °C are egg-spawning groups and temperatures at 18.5 °C and 18 °C are non-spawning groups. (C) Validation of RNA−seq data with quantitative RT−PCR (qPCR). Log2 fold changes of RNA−seq data for gene expression were plotted against those of qPCR data. The reference straight line in black indicates the linear relationship between the results of RNA−seq and qPCR. (p < 0.00001, correlation coefficient = 0.8114). (D) Fold changes and standard error of mean (SEM) for 17 selected genes of RNA−seq were validated with qPCR.

Acknowledgments

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