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Fig. 2

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ZDB-IMAGE-220726-86
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Figures for Boger et al., 2022
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Figure Caption

Fig. 2

The loss of Elmo1 and Elmo3, respectively, led to an increased diameter of hyaloid blood vessels in zebrafish larvae. (A) Quantification of larval hyaloid vasculature at 120 hpf showed no changes in branches and sprouts but an increase in the vessel diameter in elmo1 −/− compared to elmo1 +/+ . n = 33–37 hyaloids per group. (B) Quantification of larval hyaloid vasculature at 120 hpf showed no changes in branches, sprouts and vessel diameter in elmo2 −/− compared to elmo2 +/+ . n = 33 hyaloids per group. (C) Quantification of larval hyaloid vasculature at 120 hpf showed no changes in branches and sprouts but an increase in the vessel diameter in elmo3 −/− compared to elmo3 +/+ . n = 27–31 hyaloids per group. (D–F) Representative confocal microscopy images of the hyaloid vasculature of elmo1 +/+ and elmo1 −/− (D), elmo2 +/+ and elmo2 −/− (E) and elmo3 +/+ and elmo3 −/− (F) zebrafish larvae at 120 hpf. Scale bar is 50 µm. Statistical analysis was done with t-test and Mann-Whitney test. *p < 0.05, **p < 0.01. ns, not significant; hpf, hours post fertilization.

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