Fig. 2

Fig. 2

Fig. 2. Inhibition of the neutrophil inflammasome leads to an increase in the number of neutrophils in zebrafish with skin inflammation. (A) Schematic showing the zebrafish lines used to inhibit neutrophil inflammasome. The offspring were screened using fluorescent green heart marker of the Tg(uas:ascΔCARD-GFP). (B, C) One-cell stage zebrafish eggs were microinjected with control or spint1a crRNA/Cas9 complexes. At 3 dpf, neutrophils (B) and erythrocytes (C) were stained with neutral red, Sudan black and o-dianisidine, respectively. ****p < 0.0001; n.s., non-significant.