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Fig. 9
Maintenance of CP epithelial polarity and junctions in cldn5a-/- larvae. A Apical polarity marker of PKCζ and epithelial junction proteins were stained to investigate the integrity of dCP epithelium. In the larval brains of wild types and cldn5a-/- with NM, PKCζ colocalizes with Cldn5a and is linearly expressed in the apical side of dCP epithelium. In cldn5a-/- with BE, PKCζ localizes non-apically and misexpresses on the protruded CP into the ventricle (white arrowhead) and the misfolded plexus epithelial layers (white arrow). Furthermore, compared to that in wild types, cldn5a-/- larvae show a dramatic upregulation of ZO-1. There was no discrepancy in the expression of Beta-catenin between wild types and cldn5a-/- mutated larvae. White dot lines figure out the linear dCP epithelium. n > 3 fishes analyzed per group. Scale bars: 20 μm. B, C The quantitative analysis of ZO-1 and Beta-catenin by measuring the mean gray values in CP epithelial cells with ImageJ software. Data are shown as mean ± SEM. ***P < 0.005, ****P < 0.001