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Fig. 1 (a) Representative photographs showing the fins before amputation, post amputation and at 1, 5 and 10 DPA in control (C) (n = 7) and probiotic-treated (P) (n = 7) groups (Scale bar = 2,000 µm); (b) Picture of a representative amputated fin, showing the regenerated area (REG, green dotted tracing), the stump width or width of the amputation plane (STU, red dotted line), the fin ray width (RAY, pink line), the segment length (SEG, yellow line) and the peduncle width (PED, blue dotted line); (c) Statistical analysis of regeneration rate (expressed as %) between C and P groups calculated at 10 DPA with respect to the fin before amputation of the same fish. (d–g) Various morphometric parameters used to analyze the regenerated areas in C and P fins (n = 7 per group per time point) (d) Regenerated area (REG)/Peduncle width (PED) ratio; (e) Regenerated area (REG)/Stump width (STU) ratio; (f) Mean ray width (RAY) and (g) Mean segment length (SEG). T-test was used to analyze the differences among the groups. % Values were converted to respective decimal values before applying T-test and statistical significance was set at p < 0.05.