ZFIN Image: Schirmer et al., 2022, Figure 4

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Figure Caption

Figure 4

MALDI MSI visualizing the lipid distribution in D. magna. (a) Whole body image of D. magna. (b) RGB Overlay of PC (36:0) [M+Na]⁺ (blue colored), HexCer (41:1;O₂) [M+2Na-H]⁺ (red colored) and SM (38:1;O₂) [M+Na]⁺ (green colored) visualizing different anatomical regions (thoracic legs, egg/embryo, intestine, body wall). (b–d) Positive ion MS images. (c) Ion image of PC (36:0) [M+Na]⁺, highlighting the intestine region (labeled by a white arrow). (d) Ion image of HexCer (41:1;O₂) [M+2N-H]⁺, highlighting the lipid distribution within the egg/embryo (labeled by a white arrow). (e) Ion image of SM (38:1;O₂) [M+Na]⁺, showing the lipid distribution in the surrounding tissue including body wall (labeled by a white arrow) and thoracic legs.

Acknowledgments

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