Figure 4—figure supplement 2.

Figure 4—figure supplement 2.

(A) Confocal z-stack images of embryos as described in Figure 4G treated with 4 μM SL327 or DMSO from 51 to 120 hours post fertilization (hpf). Scale bar: 100 μm. (B) Confocal z-stack image of embryos treated with DMSO or SL327 as described in Figure 4G. Embryos were fixed at 3 days post fertilization (dpf) and used for TUNEL (cyan) and α-DsRed staining (grey). Zoom-in single slice images of TUNEL staining in merged and TUNEL channel showing the colocalization of the signal. Scale bar: 100 μm, 30 μm (enlarged images). (C) Corresponding quantification of TUNEL signal from (C). In SL327-treated group (n = 45), lymphatic endothelial cell (LEC) showed a positive TUNEL staining rate of 15.56% while it was 0% in DMSO-treated group (n = 45).