Figure 3—figure supplement 3.

Figure 3—figure supplement 3.

(A) Illustration of FACS and qPCR analysis on 3 days post fertilization (dpf) Tg(prox1a:TagRFP; fli1a:nGFP) embryos. (B) (Left) Gating strategy for FACS sort of Tg(prox1a:TagRFP; fli1a:nGFP) as described in Figure 3—figure supplement 3A. Sorting was performed on all singlet, alive cells according to their expression of DsRed (red, 561 nm) and GFP/ YFP (green, 488 nm). (Right) RT-qPCR of cxcr4a, cxcr4b, and ackr3b expression in trunk LECs at 3 dpf. Graph represents gene expression relative to geometric average of kdrl and β-actin from three biological repeats (mean ± SEM). Unpaired two-tailed Student’s t-test or Mann-Whitney test was used. *p < 0.05, **p < 0.001. Ns, no significance. (C) Gating strategy for FACS sort of Tg(flt1:YFP) and Tg (abcc9:Gal;UAS:GFP) as described in Figure 3D. Sorting was performed on all singlet, alive cells according to their expression of GFP/ YFP (green, 488 nm).