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Figure 3—figure supplement 1. Mural cells express chemokines and growth factors.

(A–D) Re-clustering of prospective mural cells from published pdgfrb:egfp scRNA-seq. (A) Uniform Manifold Approximation and Projection (UMAP) plot of previously described clustering for cells isolated from TgBAC(pdgfrb:egfp) cells at 5 days post fertilization (dpf). Highlighted cells are those used for re-clustering. Adjacent text summarizes steps used for re-clustering. See Materials and methods section of additional details. (B) UMAP plot following analysis of cell highlighted in (A). (C) Violin plot showing markers used to assign cell identity to clusters identified in (B). (D) Violin plot showing expression of genes encoding receptors for Vegfc and Cxc12. Also shown are definitive markers for perivascular fibroblasts identified in previous studies.

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