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Figure 1—figure supplement 2. Trunk of 5 days post fertilization (dpf) control and PDGFRβ inhibitor-treated embryos.

(A) Confocal z-stack images of 5 dpf Tg(fli1a:nEGFP);(–5.2lyve1b:DsRed2) treated with 20 μM PDGFR inhibitor AG1296 (n = 15) or DMSO (n = 15) from 48 hours post fertilization (hpf). Scale bar: 100 μm. (B) Quantification of total lymphatic endothelial cell (LEC) number in (C). Data are presented as mean ± SEM, Mann-Whitney test was used. ***p < 0.0005.

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