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Fig. 5.
Estrogens promote inhibitory synaptogenesis in the OB. (Ai,Aii) A transverse cryosection image of EROB cells (green), gephyrin puncta (red) and nuclei (blue) in EE2-exposed 4 dpf ERE:GFP embryos. The distal-ends of EROB cell projections co-localise with (arrowheads) or localise in close proximity to (arrows) gephyrin+ inhibitory synapses at an olfactory glomerulus. Aii shows a magnified image of a sub-region of olfactory glomerulus in Ai. (Bi-Dii) Estrogens promote inhibitory synaptogenesis in the OB, whereas ICI inhibits the formation of inhibitory synapses. (Bi,Bii) control; (Ci,Cii) EE2-treated; (Di,Dii) ICI-treated 4 dpf ERE:GFP embryos. Gephyrin puncta (red) in the OB are shown with arrowheads. Bii, Cii and Dii show magnified images of a sub-region of the OB indicated in Bi, Ci and Di, respectively. (E) Percentage increase in the total numbers of gephyrin+ puncta in the OB. The number of gephyrin+ puncta was quantified for three different sizes, 0.1-0.4 µm2 (white), 0.41-0.99 µm2 (grey) and 1-10 µm2 (blue). Data are mean±s.e.m., n=4. *P<0.05, **P<0.01, ***P<0.001 (ANOVA with Tukey post-hoc test).