ZFIN Image: Tang et al., 2022, Figure 2

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Figure 2

Assessment of the effect of IS on cardiac development in zebrafish embryos. (A) Morphological changes in the heart. The results showed that IS exposure causes cardiac dysmorphology and pericardial edema in zebrafish embryos. Furthermore, the atria and ventricles of the heart were elongated and separated. Morphology was assessed visually using a stereomicroscope (Leica MZ10 F). (B) Tg(fli1:GFP) zebrafish embryos were treated with 2.5 mM IS. (C) HE staining. Zebrafish embryos treated with 2.5 mM IS were fixed in 10% formalin for one day, immersed in 1% agarose, paraffin-embedded and stained with HE. The stained tissues were observed under a microscope. The dotted lines indicate the developing heart. Compared to the control condition, IS exposure caused elongation of the heart and distal separation of the ventricle and atrium. (D) Representative results for ROS production in pericardial tissues using DHE staining. The white arrows indicate the signals where ROS accumulated in the pericardium. Control embryos were soaked in egg water (60 μg/mL sea salt) without IS treatment.

Acknowledgments

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