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Figure 8
Capsule on the cell surface decreases in the absence of LCP proteins. (A) Percoll buoyant density centrifugation revealed less capsule in ΔCpsA, ΔPsr and ΔCpsA-ΔPsr strains in comparison to WT GBS. (B) Dot blot analysis on supernatants of WT, ΔCpsA, ΔPsr and ΔCpsA-ΔPsr strains reveals that more supernatant is released into the supernatant of ΔCpsA and ΔCpsA-ΔPsr strains. Significance was determined by comparing densities of each strain to that of wild type GBS. Values represented are averages of three biological replicates. * p < 0.05.
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