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Figure 4
P. pentosaceus YC contributes to NLRP3 inflammasome activation after a 4-week administration. Differentially expressed genes (CON vs. YC4W, YC1W vs. YC4W) of zebrafish intestine were analyzed and used for pathway classification based on KEGG enrichment analysis. (A) Immune-related pathways changed in both comparison CON vs. YC4W and YC1W vs. YC4W. (B) Heatmap of differentially expressed genes of C-type lectin receptor signaling pathway and NOD-like receptor signaling pathway in comparison to CON vs. YC4W. (C) Differentially expressed intestinal genes in comparison to CON vs. YC4W and relative nlrp3 mRNA quantification of zebrafish intestine by qPCR (n = 6). (D) ELISA analysis of intestinal IL-1β level of zebrafish from CON, YC1W, and YC4W groups (n = 6). Representative (E) immunohistochemical and (F) Western blot analysis of intestinal IL-1β of zebrafish from CON, YC1W, and YC4W groups. Scale bars represents 20 µm. Data are represented as mean ± SEM. *p < 0.05 by one-way analysis of variance followed by Dunnett’s multiple comparisons test.