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FIGURE 1
Generation of atrn mutation in zebrafish by CRISPR-Cas9 strategy, and the average velocity of the male mutant is faster than the wild type during the day, but in the dark, the female mutant likes to stay quiet. (A) Zebrafish attractin has the CUB domain, kelch-type beta propeller, C-type lectin-like domain, and laminin EGF domain. (B) Exon 8 is the target for CRISPR/Cas9 gene editing in zebrafish atrn. The CRISPR/Cas9-induced mutation (22-basepair deletion) in atrn is shown in annotated atrn mutant sequences. (C) The mutated atrn mRNA with PTC was predicted to encode truncated protein. (D) Locus diagram of male and female in sibling atrn+, sibling atrn+/–, and sibling atrn–/– groups at 4 mpf during daytime. Average velocity in 1 h was recorded in three genotype groups at 4 mpf. At 10:00–14:00, we analyzed the tracking data of male (E) and female (F) three-genotype groups; the average velocity of the male mutant is faster than the male wild type, but there is no significance among the three female groups. (G) Locus diagram of male and female in sibling atrn+, sibling atrn+/–, and sibling atrn–/– groups at 4 mpf at night. At 00:00–4:00, we analyzed the tracking data of male and female three genotype groups. The average velocity of the female mutant is slower than the male wild type (H), but there is no significance among the three male groups in the night (I). N = 10. Data are shown as mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001.