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Fig. 7 A dominant-negative Rbpj blocks expression of tbxta. (A) Embryos were heat shocked at the 10-somite stage and left to develop. The dominant-negative Rbpja (EnR-Rbpja) causes posterior defects. (B-J) Embryos were heat shocked at the 10-somite stage and fixed 5 h post-heat shock. Expression of tbxta in the tailbud is inhibited by EnR-Rbpja (D) (100%, n=19) but not by Rbpja (B,C) (100% for each, n=20 WT and 16 Rbpja), whereas the notochord expression is not inhibited (red arrowhead). The expression of cdx1a and snai1a are not inhibited by EnR-Rbpja (G,J) (100% in all cases, n=minimum of 14 embryos of each type).