FIGURE 2

FIGURE 2

Smc1b mutant zebrafish display defects in spermatogenesis. (A)Smc1b mutations used in this study: smc1b^Q198X located in exon 4 and smc1b^Q261X in exon 5. Both mutations cause premature termination codons. (B–E) Hematoxylin-eosin (H&E) staining on adult testes of wild type (B), smc1b^{Q261X(–/–)}(C), smc1b^{Q198X(–/–)}(D), and smc1b^Q198X/Q261X(E). Dashed lines denote examples of: spermatogonia (yellow), spermatocytes (red), and spermatozoa (orange). (F,G) Caspase-3 (green) and DAPI (blue) staining on adult testes from wild type (F) and smc1b mutant (G). Caspase-3 positive cells are indicated by arrows. (H) Quantification of caspase-3 positive cells per testis area in the mutant (N = 3 testes) and wild type (N = 3 testes). Student’s t-test shows no significant (p = 0.3524) difference between wild-type and mutant caspase-3 positive cells numbers. Scale bars are 50 μm.