FIGURE 4

FIGURE 4

Summary of the projection paths of individual inhibitory efferent cells. (A–C) Schematic zebrafish larvae showing the cumulative projection paths of REN (n = 8), ROLE (n = 10), and RELL (n = 9) cells. Left drawing illustrates the projection paths of the REN, ROLE, and RELL cells (highlighted in magenta) within the rhombencephalon; projections of the sensory ganglion into the brain are shown in cyan. For each inhibitory efferent the cumulative projection path across the lateral line is highlighted (magenta) in the schematic zebrafish larvae on the right (for individual processes, please see Supplementary Figure 3). Identity of innervated neuromasts is listed below each schematic (left: anterior lateral line neuromasts; right: posterior lateral line neuromasts). (D) Quantification of the number of neuromasts connected revealed no significant differences between REN, ROLE, and RELL cells (K = 2.789, p = 0.25). The total length of the projections made by the RELL cells was significantly longer compared to that of the REN and ROLE cells (K = 18.59, p < 0.0001). (E) No significant differences were found between the number of neuromasts connected by individual inhibitory efferent cells that project to the ALL only, ALL and PLL, or PLL only (K = 5.609, p = 0.06). The total length of the projections made by cells that connect in the PLL was significantly longer compared to those connecting to the ALL but not the ALL+PLL (K = 16.84, p < 0.0001). Age of larvae: 5dpf. ALL, anterior lateral line; PLL, posterior lateral line; REN, the rostral efferent nucleus; CEN, the caudal efferent nucleus nucleus; ROLE, the rhombencephalic octavolateral efferent neuron; RELL, the rhombencephalic efferent neuron to the lateral line.