IMAGE

Figure 5

ID
ZDB-IMAGE-210617-63
Source
Figures for Ceriani et al., 2021
Image
Figure Caption

Figure 5 POA generates new cells with 8-day pulse-chase labeling. (A–J) BrdU/PCNA double immunofluorescence in transverse paraffin sections of 5 µm. (A–E) Treatment with one pulse of BrdU on day 1 and brains fixed on the second day. (F–J). Treatment with two pulses of BrdU at 1 and 7 days, and brains were fixed at day 8. All sections were labeled with DAPI (blue). Scale bar: 30 μm. (K,M) Percentage of cells double or single labeled for BrdU or PCNA located in the POA, with single-BrdU pulse on day 1 (K) and two BrdU pulses at 1 and 7 days (M). (L,N) Percentage of cells double or single labeled for BrdU or PCNA in representative sections of the POA with single-BrdU pulse on day 1 (L) and two BrdU pulses at 1 and 7 days (N). For each representative region of the POA, the number of sections (L) (PPa1 = 5, PPa2 = 10, PPa3 = 13, PPa4 = 12, and PPp1 = 4) and (N) (PPa1 = 5, PPa2 = 10, PPa3 = 13, PPa4 = 12, and PPp1 = 4) labeled, BrdU+, PCNA+ and BrdU+/PCNA+ cells were counted. Graphs plotted with SEM.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.