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Figure 1 Anatomy of the preoptic area (POA) in adult zebrafish. (A) Schematic representation of zebrafish brain. The red, boxed area indicates the POA region. (B–I) Transverse paraffin sections of the POA stained using trichrome stain. POA region starts from the commis-sure anterior to the optic chiasm. Each slice corresponds to the representative region of the POA, categorized with a code: PPa0, PPa1, PPa2, PPa3, PPa4.1, PPa4.2, PPp1, and PPp2, where the numbers indicate the distance in μm from the beginning of the POA. (G) Cells with a fusiform nucleus are observed in the ventral region of the PPa (arrow in the boxed area). Commissure, pars dorsalis (Cantd); anterior commissure, pars ventralis (Cantv); diencephalic ventricle (DiV); anterior part of the parvocellular preoptic nucleus (PPa); posterior part of the parvocellular nucleus (PPp); optic nerve (ON); suprachiasmatic nucleus (SC); magnocellular nucleus (PM). Scale bar: (I) 50 μm, (G) in r.ectang.le 10 μm.