ZFIN Image: Brunt et al., 2021, Fig. 2

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Figure Caption

Fig. 2 Vangl2 controls the emergence of Wnt8a cytonemes in fibroblasts.

A–J Wnt8a-positive PAC2 zebrafish fibroblasts transfected with indicated constructs, imaged and analysed live 24 h of post-transfection. Yellow arrows indicate examples of Wnt8a positive cytonemes. Scale bar = 10 µm. K Number of Wnt8a positive cytonemes per cell (n = number). (n per condition = 25, 9, 14, 6, 25, 14, 31, 36, 13 cells). L Length of Wnt8a positive cytonemes in PAC2 cells (µm). (n per condition = 139, 52, 32, 21, 131, 65, 47, 51, 11 cytonemes). M Breakdown of the percentage of Wnt8a positive cytoneme lengths into 0–5, 5–10, 10–15, 20+ µm categories. Graphs represent mean and standard error of the mean. K, L Two-sided Kruskal–Wallis tests with Bonferroni correction for multiple tests. Statistical significance: p ≤ 0.05. SEM = 1. Corresponding dot plots are shown in Supplementary Fig. 2, and analysis of PAC2 filopodia is shown in Supplementary Figs. 3 and 4. Source data are provided as a Source Data file.

Acknowledgments

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