ZFIN Image: Duong et al., 2021, Fig. 8

Image

Figure Caption

Fig. 8 Pacemaker CMs do not differentiate in RA-deficient embryos. (A,B) IHC of Isl1 (green), Mhc (red), Amhc (blue) in hearts of DMSO- and DEAB-treated embryos at 48 hpf. Number of embryos examined: DMSO-treated n = 25, DEAB-treated n = 21. Arrows in A and B indicate Isl1 expression and the lack of Isl1 at the venous poles of the hearts in control and DEAB-treated embryos, respectively. (C,D) IHC of GFP (green), Mhc (red), Amhc (blue) in hearts of DMSO- and DEAB-treated sqet33-mi59B embryos at 48 hpf. Number of embryos examined: DMSO-treated n = 11, DEAB-treated n = 11. Arrows in C and D indicate the sqet33-mi59B GFP expression and its reduced expression at the venous poles of the hearts in control and DEAB-treated embryos, respectively. The remaining sqet33-mi59B expression in DEAB-treated embryos is adjacent to the atrial CMs. Views are ventral with anterior up. Scale bar – 50 μm.

Acknowledgments

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Reprinted from Developmental Biology, 473, Duong, T.B., Holowiecki, A., Waxman, J.S., Retinoic acid signaling restricts the size of the first heart field within the anterior lateral plate mesoderm, 119-129, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.