FIGURE 3

FIGURE 3

Formation of chromosomal axes in sycp1 mutant spermatocytes. Immunostaining of Sycp3, Sycp2 and Sycp1 components in wild-type (sycp1^+/+, i to iii) and sycp1^isa/isa (iv to vii) spermatocyte chromosome spreads. Wild-type nuclei at leptotene (L, i), mid-zygotene (MZ, ii) and pachytene (P, iii) were staged according to the staining patterns of Sycp3, Sycp2 and Sycp1 (Blokhina et al., 2019). sycp1^isa/isa nuclei at leptotene (L, iv), mid-zygotene-like (MZ-like, v) and late zygotene/pachytene-like (LZ/P-like, vi) stages were staged by axis staining patterns. Regions indicated in white rectangles are shown at the bottom at a higher magnification.