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Figure 5—figure supplement 4.

ID: ZDB-IMAGE-210319-27
Source: Figures for Vinayagam et al., 2020

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Figure Caption

Figure 5—figure supplement 4. Cryo-EM image processing and structure determination of TRPC4 solubilized in LMNG.

(A) The top left panels show a representative micrograph and class averages of TRPC4 solubilized in LMNG. Scale bars, 50 nm and 10 nm, respectively. The density resulting from the initial 3D refinement is shown in the top right panel. (B) Angular distribution of particles used in the final refinement and Fourier shell correlation curves (FSC) between the two independently refined maps. The dotted lines indicate the 0.143 FSC criterion used for average resolution estimation. (C) The final map, filtered using LAFTER, shows a clear density corresponding to a horizontal helix (indicated with an arrowhead) (left panel). The corresponding structure in cartoon representation with the residues of the horizontal helix shown in golden yellow (middle panel). Zoom-in view of the horizontal helix (right panel).

Acknowledgments

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