Image
Figure Caption

Figure 5—figure supplement 1. Analysis of CaM binding to TRPC4 by biochemical methods.

(A) SDS gel electrophoresis analysis of the TRPC4 pull down experiment performed with a CaM Sepharose column. Lane 1 - protein size marker, lane 2 - TRPC4 input, lane 3 - flow through, lane 4 - wash, remaining lanes - elution fractions. (B) Gel filtration analysis of the TRPC4-CaM complex. The peak fraction containing the TRPC4-CaM complex is indicated by a star were further analyzed by SDS gel electrophoresis (right panel).

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