Figure 4—figure supplement 2.

Figure 4—figure supplement 2.

(A) Close-up view of the Ca²⁺-binding site in apo and ligand-bound TRPC4 determined in amphipols. The coordinating residues in the Ca²⁺ ion binding site and the modelled Ca²⁺ ion are shown in stick and sphere representation along with their densities. The oxygen atom of the ligand molecules is situated close to the bound Ca²⁺-ion (refer)Figure 4C (B) Close-up view of the Ca²⁺-binding site in apo and TRPC4-CaM complex determined in presence of lauryl maltose neopentyl glycol (LMNG). The external addition of Ca²⁺ ion during the TRPC4-CaM complex preparation reflects its strong density. The different structural forms shown in (A) and (B) are color coded and named accordingly.