Figure 2—figure supplement 1.

Figure 2—figure supplement 1.

(A) The top panel shows a representative digital micrograph area and selected 2-D class averages of inhibitor GFB-8438 bound TRPC4. Scale bars, 50 nm and 10 nm, respectively. The initial refinement density and subsequent densities obtained after 3D classification are shown next to the 2-D class averages and in the bottom panel, respectively. (B) Angular distribution of particles used in the final refinement and Fourier shell correlation curves (FSC) between the two independently refined maps. The dotted lines indicate the 0.143 FSC criterion used for average resolution estimation. (C, D) Same as in (A) and (B), respectively, but for the inhibitor GFB 9289-TRPC4 complex. (E, F) Similar to (A) and (B), respectively representing inhibitor GFB-8749-TRPC4 complex.