ZFIN Image: Prentzell et al., 2021, Figure 6

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Figure Caption

Figure 6

G3BP1 suppresses mTORC1-driven migration in breast cancer cells

(A) Scratch assay with shG3BP1 #1 cells. Scale bar, 150 μm. n = 3.

(B) Quantitation of data in (A). Shown are data points and mean ± SEM.

(C) Real-time cell analysis (RTCA) of proliferation of shG3BP1 #1 MCF-7 cells. Mean ± SEM. n = 6.

(D) Quantitation of data in (C). Shown are data points and mean ± SEM.

(E) Transwell migration of G3BP1 KO cells (6–8 h). Scale bar, 150 μm. n = 5.

(F) Quantitation of data in (E). Shown are data points and mean ± SEM.

(G) G3BP1 mRNA expression. Expression values from The Cancer Genome Atlas (TCGA) processed and normalized by RNA-Seq by Expectation Maximization (RSEM) are classified according to PAM50. Data are shown as boxplots, median with 25^th+75^th percentiles as boxes, and 5^th+95^th percentiles as whiskers.

(H) Relapse free survival (RFS) of individuals with breast cancer based on G3BP1 RNA levels.

(I) RFS of individuals with breast cancer based on G3BP1 protein levels.

(J) RFS of individuals with breast cancer based on TSC1 RNA levels.

(K) RFS of individuals with breast cancer based on TSC2 RNA levels.

Acknowledgments

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Reprinted from Cell, 184(3), Prentzell, M.T., Rehbein, U., Cadena Sandoval, M., De Meulemeester, A.S., Baumeister, R., Brohée, L., Berdel, B., Bockwoldt, M., Carroll, B., Chowdhury, S.R., von Deimling, A., Demetriades, C., Figlia, G., Genomics England Research Consortium, de Araujo, M.E.G., Heberle, A.M., Heiland, I., Holzwarth, B., Huber, L.A., Jaworski, J., Kedra, M., Kern, K., Kopach, A., Korolchuk, V.I., van 't Land-Kuper, I., Macias, M., Nellist, M., Palm, W., Pusch, S., Ramos Pittol, J.M., Reil, M., Reintjes, A., Reuter, F., Sampson, J.R., Scheldeman, C., Siekierska, A., Stefan, E., Teleman, A.A., Thomas, L.E., Torres-Quesada, O., Trump, S., West, H.D., de Witte, P., Woltering, S., Yordanov, T.E., Zmorzynska, J., Opitz, C.A., Thedieck, K., G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling, 655-674.e27, Copyright (2021) with permission from Elsevier. Full text @ Cell