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Fig. 1 Quantitative Analysis of Microglia Morphology in Evolutionarily Distant Animals (A) Phylogenetic tree based on the NCBI taxonomy of animals used in this study (generated via https://phylot.biobyte.de/). Ma, million years. (B–G) Three-dimensional reconstruction (B; scale bars represent 20 μm) and Imaris-based automatic quantification (C–G) of the cell morphometry of cortical Iba1+, Mpeg1-EGFP+ (zebrafish), and CSF1R-mApple+ (chicken) microglia. Each panel displays one individual sample with at least three measured cells per animal. Data are presented as mean ± SEM. (H) Brain tissue was subjected to immunohistochemistry for Iba1 or stained with antibodies against the respective fluorescent proteins (zebrafish and chicken) to detect microglia. Scale bars represent 50 μm. Representative images are displayed. (I) Number of ramified parenchymal microglia in the CNS. (J) Number of cresyl violet+ cortical neurons in the CNS. (K) Calculated ratio of neurons per microglia. For (H)–(K), each symbol displays one individual sample. Three to four sections per sample were analyzed. Data are presented as mean ± SEM.
Reprinted from Cell, 179, Geirsdottir, L., David, E., Keren-Shaul, H., Weiner, A., Bohlen, S.C., Neuber, J., Balic, A., Giladi, A., Sheban, F., Dutertre, C.A., Pfeifle, C., Peri, F., Raffo-Romero, A., Vizioli, J., Matiasek, K., Scheiwe, C., Meckel, S., Mätz-Rensing, K., van der Meer, F., Thormodsson, F.R., Stadelmann, C., Zilkha, N., Kimchi, T., Ginhoux, F., Ulitsky, I., Erny, D., Amit, I., Prinz, M., Cross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program, 1609-1622.e16, Copyright (2019) with permission from Elsevier. Full text @ Cell