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Figure 7 TTO measurements in fga morpholino knockdown embryos with transgenic expression of AαE, AαE R28C, or both, to model dysfibrinogenemia. (A) A scheme representing the experimental set-up. Early TU zebrafish embryos were injected with and without an fga mRNA-targeting morpholino (MO) in the absence or presence of Tol2 plasmids for expression of fibrinogen AαE, AαE R28C, AαE + AαE R28C or a half quantity of AαE (1/2 AαE). Tol2 transposase mRNA was also present within injection mixes (not shown). At 3 dpf the TTO assay was performed to measure venous thrombosis time. The MO inhibits endogenous fga mRNA splicing but cannot target the transgenically expressed AαE and AαE R28C mRNAs. (B) TTO data for each experimental group, each circle represents one larva (n = 19 to 22).