Fig. 4

Fig. 4

Imaging larval zebrafish: (A and B). Schematic of a single light sheet covering the hindbrain of a larval zebrafish from above (A) as well as a number of light sheets (constituting a volume) from the side (B, sheet size and spacing are not to scale). (C) Transgenic zebrafish expressing the calcium reporter GCaMP6f in the cell nuclei of all neurons (Tg(elavl3:H2B-GCaMP6f)) were embedded in agarose and positioned so that the laser entered the brain from the side (blue arrows in A). Representative sections of the volume taken at 20, 30 and 40 μm depths at a frequency of 1.6 volumes/sec and an integration time of 10.2 ms per section. The step size was 2 μm, hence representative sections are 5 sections apart. The laser was set to 1.8 mW. (D) magnified view of boxed areas in C. Single cell nuclei are clearly visible and three examples are highlighted. (E) Activity of single cells highlighted in D.