Figure 2

Figure 2

Mouse 3T3-L1 preadipocyte differentiation assay identified ten lipid-lowering NPs. (a) Experimental design for 3T3-L1 assay. (b) Result of 3T3-L1 screening. NPs without data (not determined: ND) were due to inability to extract compounds with ethanol extraction. Values presented are means. Error bars indicate SD. n = 8, * p < 0.05, ** p < 0.01 vs. control l, one-way ANOVA with Bonferroni–Dunn multiple comparison. (c) Representative images of differentiated 3T3-L1 cells with the same NPs as in Figure 1d. Red indicates lipid accumulation. (d) Venn diagram analysis of the effective NPs in both the ZOT (Figure 1) and 3T3-L1 cell-based assay. * While NP01 (globin digested peptides [GDP]) could not be evaluated in the 3T3-L1 assay, NP02 (GDP with magnesium) reduced lipid accumulation in 3T3-L1 cells. Thus, we categorized NP01 as effective in both assays.