Figure 1

Figure 1

Zebrafish obesogenic test (ZOT) identifies seven anti-obese natural products (NPs). (a) Experimental design for ZOT. (b) Short term high-fat diet (HFD) increased the amount of visceral adipose tissue (VAT). Right panel indicates typical images of the control and HFD groups. Red indicates Nile Red (NR)-stained VAT. sb; swimming bladder, vat; visceral adipose tissue. Left panel indicates quantification of NR stained area. Values presented are means. Error bars indicate SD. n = 5, ** p < 0.01, Student’s t-test. (c) ZOT result. Dotted lines indicate 1 and 0.6 ratio to HFD alone group (HFD). NPs with asterisks were administered at lower volumes (10 µg/mL) because of their toxicity. The others were administered at 100 µg/mL in the breeding water. Values presented are means. Error bars indicate SD. n = 5–10, * p < 0.05, ** p < 0.01 vs. control, one-way ANOVA with Bonferroni–Dunn multiple comparison. nd indicates not determined because of high toxicity (NP18). (d) Typical image of effective NPs in (c).