Figure 5—figure supplement 4.

Figure 5—figure supplement 4.

(A) Maximum likelihood phylogeny of selected poriferan and bilaterian Eomesodermin sequences. The multiple sequence alignment consists of 37 sequences aligned over 434 positions (proportion of gaps and undetermined characters: 22.80%). Sequences were downloaded from uniprot.org or taken from NCBI (gi_*). TBX4 and Brachyury sequences serve as outgroups because they are most closely related to the Eomes family according to Sebé-Pedrós et al., 2013 and HMM-HMM searches (Supplementary file 1–Supplementary Table 14). A phylogenetic analysis with an identical dataset, including the two poriferan Eomes candidates (highlighted in red; from Sebé-Pedrós et al., 2013), is presented in panel B (39 sequences aligned over 435 positions; proportion of gaps and undetermined characters: 23.64%). Branch labels correspond to the results of SH-aLRT (Shimodaira–Hasegawa-like approximate likelihood ratio test, left) and UFBoot (ultrafast bootstrap approximation, right) as implemented in IQ-TREE (Nguyen et al., 2015). Tree topology and corresponding bootstrap values do not clearly assign the poriferan sequences to the Eomes family of T box proteins.