Figure 5—figure supplement 2.

Figure 5—figure supplement 2.

Maximum likelihood phylogeny of selected bilaterian Lefty and Nodal proteins. The corresponding multiple sequence alignment consists of 24 sequences with 446 columns and 29.01% gaps and undetermined characters. The sequences correspond to OG_11821 (Lefty) and OG_12210 (Nodal) of the original clustering plus several additional candidate sequences from public repositories (red dots). Blue dots highlight whether a sequence is derived from transcriptomic (light blue) or genomic ORF data (dark blue). All other sequences can be accessed at NCBI with the gene identifiers given as branch labels. Blue triangles identify previously described Lefty and Nodal reference sequences. Bootstrap values below 50% are removed for clarity. There are three Nodal-related genes in teleosts, cyclops, squint, and southpaw, as a result of lineage-specific duplications (Fan and Dougan, 2007).