ZFIN Image: Dai et al., 2014, Fig. S4

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Figure Caption

Fig. S4

Low [Ca2+] treatment or IGF1R inhibition has no effect on pErk signaling in larval zebrafish. (a) Lack of effect of low [Ca²⁺] treatment on pErk levels. 72 hpf zebrafish larvae were transferred to artificial water with the indicated [Ca²⁺], raised to 120 hpf, fixed and stained for pErk. Inserts are higher magnification views. Tr, trunk; Pe, pectoral fin; Yo, yolk sac. Scale bar = 50 μm. (b and c) The pErk signal is independent of IGF1R. 72 hpf larvae were transferred to the 0.001 mM [Ca²⁺] water containing DMSO, BMS-754807 (0.6 μM), NVP-AEW541 (6 μM), U0126 (10 μM), or PD98059 (10 μM). After 8 h, they were stained for pErk. Representative views are shown in (b) and quantitative results are shown in (c). The animals were scored according to the scoring system shown in Fig. S1b. The number of fish analyzed is shown on the top of each column.

Acknowledgments

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