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Fig. 6 S1
Assessment of visuomotor behaviours in control larvae.
(A) RGC axonal arborisations in AF7 (magenta) before (top, 6 dpf) and 24 hr after (bottom, 7 dpf) bilateral laser-ablation of KalTA4u508 pretectal neurons (green) in a KalTA4u508;UAS:mCherry;atoh7:GFP larva. (B) Sham ablations. Thalamic neurons before (top, 6 dpf) and 24 hr after (bottom, 7 dpf) bilateral laser-ablations in a atoh7:GFP;gata2a:GFP larva. Images show maximum-intensity projections (20 µm depth). Targeted cells are outlined in yellow. (C) Mean number of cells labelled in the thalamus per hemisphere before and after ablations (N = 10 fish). (D) Mean number of ablated thalamic cells per hemisphere. (E) Paramecia consumption in atoh7:GFP;gata2a:GFP larvae, before and after thalamic ablations (N = 10 fish). Black symbols show mean ± SEM across animals. (F–H) Non-ablated control. Assessment of Paramecia hunting in KalTA4u508;UAS:mCherry;elavl3:itTA;Ptet:ChR2-YFP control larvae at 6 and 7 dpf (N = 16 fish). (I–L) Average swim speed, loom-evoked escape and OMR, in control larvae. (M) Hunting response probability upon optogenetic stimulation of avOT in non-ablated control larvae. Scale bars, 50 µm, except in (A), 20 µm. A, anterior; L, left; P, posterior; R, right.