Sequence of PCR junction fragments amplified from genomic DNA from F1 transgenic zebrafish adults generated by GeneWeld short homology directed targeted integration.
Precise integration at the 5’ and 3’ ends in F1 progeny from F0 founder fish targeted at tyr, esama, flna, msna, aqp1a1, and aqp8a1. noto F1 progeny from founder #1 had a precise 5’ junction and imprecise 3’ junction. noto F1 progeny from founder #2 had a 5’ precise junction; no 3’ junction was amplified by PCR. Lowercase letters represent ‘padding’ nucleotides to place the integrated GTag cassette in frame with the targeted gene. Red letters represent mismatches unless otherwise noted below. esama F1 3’ junctions contain a single nucleotide variant shown in red. One esama F1 3’ junction included a 20 bp insertion (strike-through).
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