Direct sequencing of 5’ junction PCR products derived from three independently targeted bulk cell populations.
(a) Direct sequencing of 5’ junction PCR products derived from three independently targeted bulk cell populations. 48 bp HMEJ homology region and remainder of genomic AAVS1 sgRNA are indicated. Genomic sequence is directly left of the 48 bp HMEJ region and vector sequence is directly to the right of the AAVS1 sgRNA cut site.
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