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Figures for Wierson et al., 2020

Figure Caption/Comments:

Figure 5—figure supplement 1. Sequences of 5’ and 3’ junction fragments from <italic>rb1</italic> exon 2–4, <italic>rb1</italic> exon 2–25, and <italic>msna</italic> exon 2–6 deletion tagged alleles in F0 injected embryos.

Detection of precise and imprecise 5’ and 3’ junction fragments in somatic tissue of F0 embryos injected with two guides that target two exons and a pGTag-Gal4/VP16 donor with 5’ and 3’ homology arms corresponding to the 5’ exon and 3’ exon target sites. Cloned PCR amplicons were sequenced from 3 individual embryos for each targeted deletion tagging experiment.

Figure Data:
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