Sequence of PCR junction fragments amplified from genomic DNA from F1 transgenic zebrafish adults generated by GeneWeld short homology directed targeted integration.
esama, flna, and msna. esama F1 3’ junctions contain a single nucleotide variant shown in red. One esama F1 3’ junction included a 20 bp insertion (strike-through). Lowercase letters represent “padding” nucleotides to place the integrated GTag cassette in frame with the targeted gene. Red letters represent mismatches unless otherwise noted below.
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