ZFIN ID: ZDB-IMAGE-200514-34
Figures for Paatero et al., 2018

Antibody:

Figure Caption/Comments:

Fig. 7 Truncation of Ve-cadherin inhibits both JBL and endothelial cell remodeling. a Images of anti-ZO1 immunostained junctions in cdh5ubs25/+ and cdh5ubs25/ubs25 embryos. Arrows point to medial site of the junction. b Quantitation of the medial and lateral junctional thickness, based on immunostaining for ZO1; cdh5ubs25/+, n = 44 junctions (17 embryos); cdh5ubs25/ubs25, n = 40 (11 embryos); wild-type n = 28 (9 embryos). Black lines are medians. Non-parametric Kruskal–Wallis statistical test was used. c Quantitation of the duration of JBL based on EGFP-UCHD signal; cdh5ubs25/+, n = 122 (8 embryos), cdh5ubs25/ubs25 n = 103 (8 embryos) and wild-type n = 43 (3 embryos). All embryos carry the UAS:EGFP-UCHD transgene Tg(fli:GFFubs3;UAS:EGFP-UCHDubs18). d–l Tg(kdrl:EGFPs843);cdh5ubs25/+ (d–g) and Tg(kdrl:EGFPs843);cdh5ubs25/ubs25 (h–k) embryos stained for VE-cadherin (rabbit antibody, green) and ZO1 (red). Individual channels are shown in inversed contrast. Both wild-type and mutant VE-cad show junctional localization (solid arrow in panels d, f, h, and j). The junctional gap in the VE-cadherin staining of a SeA in a mutant embryo (cdh5ubs25/ubs25) is marked with dashed double arrow in panel h. l Quantification of the length of junctional gaps in control (cdh5 ubs25/+, n = 72 gaps, 23 embryos) and mutant (cdh5ubs25/ubs25, n = 139 gaps, 33 embryos) embryos. Black lines are medians. Non-parametric Mann–Whitney statistical test was used. m Still images from a confocal time-lapse of endothelial nuclei (Tg; kdrl:nlsEGFPubs1) in wild-type or cdh5ubs25/ubs25 embryos during SeA formation. T tip cell, S1 stalk cell 1, S2 stalk cell 2; double-headed arrow indicates the distance of S1 and S2 nuclei. n Quantification of movement of stalk cell 1 (S1) nuclei in relation to stalk cell 2 (S2) nuclei during cell rearrangements in SeA; cdh5ubs25/+, n = 22 SeA (4 embryos); cdh5ubs25/ubs25, n = 17 SeA (4 embryos); wild-type n = 20 SeA (4 embryos). Black lines are medians. Non-parametric Kruskal–Wallis statistical test was used. Scale bars 5 µm (a), 10 µm (d, h, m)

Figure Data:
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.