IMAGE

Fig. 5

ID
ZDB-IMAGE-200414-5
Source
Figures for Ward et al., 2019
Image
Figure Caption

Fig. 5 Sunitinib malate improves hyaloid vessel patterning, reduces retinal neovascularisation and inhibits excessive development of the choriocapillaris in vhl−/− larvae. A) Treatment from 58 hpf does not significantly increase vhl−/− primary hyaloid vessel number at 5 dpf. B) Representative images highlighting an overall improvement of vessel patterning following treatment with sunitinib from 58 hpf. White arrows denote central point from which primary blood vessels are counted. n = 3, 12 technical replicates per group per experiment. C) Reduction of ectopic intraretinal vasculature following treatment with 1 μM sunitinib. White arrows highlighting ectopic intraretinal vessels. Quantification of n = 5 larvae per treatment group. Scale bar = 20 μm. D) Z-stack projections of the sub-retinal choriocapillaris vasculature (endothelial cells shown in green) of Tg(fli1:EGFP) or vhl−/−(fli1:EGFP) at 120 hpf following treatment for 72 or 48 h as indicated. Yellow arrowheads point to interstitial pillars (ISPs). E) Quantification of the number of interstitial pillars per area of interest (AOI). n = 10 larvae.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 457(2), Ward, R., Ali, Z., Slater, K., Reynolds, A.L., Jensen, L.D., Kennedy, B.N., Pharmacological restoration of visual function in a zebrafish model of von-Hippel Lindau disease, 226-234, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.