High-throughput scRNA-seq strategy from the developing hindbrain. (A) The hindbrain of 16 hpf (pink), 24 hpf (green) and 44 hpf (blue) embryos was collected for scRNA-seq. (B) Drawing of zebrafish hindbrain with a closer view of the stereotypical hindbrain cell composition at 44 hpf. Progenitors and radial glia cell bodies occupy the ventricular region, while differentiating progenitors and neurons are in the mantle zone. (C) Schematic of the 10X Genomics Chromium workflow.
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