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Figure 3

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ZDB-IMAGE-200406-277
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Figures for Mateus et al., 2020
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Figure Caption

Figure 3

Scaling of BMP Signaling Gradients

(A and B) BRE:GFP gradients at different times as a function of the distance to intensity maxima (s=0, ROI midline). Bold lines, exponential fits.

(C and D) Decay length versus ROI half-length (L) of BRE:GFP gradients. Blue dots, individual data; black dots, average from length bins ± SEM. Slope (φ) values ± SEM are shown. Lines, linear fits with goodness of fit: φAR2 = 0.58; φPR2 = 0.55.

(E and F) Average dynamics of gradient amplitude (C0; SPIM imaging). Orange, growth phase (48–78 hpf).

(G) Log-log plot of normalized average amplitude C0(t)/C0(45hpf) versus normalized endoskeletal disc PD length L(t)/L(45hpf), to estimate q (slope value), with SEM. Power-law fit with goodness of fit (R2).

Shadowing corresponds to SEM per time point (E, F) or length (G). n represents number of fins analyzed. BRE:GFP transgene used: BRE:eGFP (Laux et al., 2011) (A–D); BRE-AAVmlp:eGFP (Collery and Link, 2011) (E–G). See also Figures S2 and S4.

Acknowledgments
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