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Figure 6
Fisetin inhibited activation of caspases 8, 9 and 3 in H9c2 cells. Caspases activity was assessed by flow cytometry using active caspase staining kits specific to caspase 8, 9 and 3, respectively. Dot plots represent the percentage of cardiomyocytes expressing activated caspase 8, 9 or 3. Normoxia: control group. HS/R: cells subjected to HS/R, treated with DMSO as vehicle control. HS/R + F: cells subjected to HS/R, treated with 15 μM fisetin. Results are expressed as the mean of three independent experiments. Two-way ANOVA. Post-hoc analysis by Tukey. **P ≤ 0.01, *P ≤ 0.05.
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