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Figure 1
The workflow of the study. Zebrafish embryos were microinjected at one-cell-stage with the desired gene construct. Two days after injection, embryos were evaluated for CFP expression. CFP positive embryos were selected to raise as F0 embryos transgenic fish. CFP positive embryos were selected and raised. CFP positive adult zebrafishes were crossed with wild type (TU strain) to produce the F1 generation.
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