ZFIN Image: Simbulan-Rosenthal et al., 2019, Figure 5

Image

Figure Caption

Figure 5

(a) Immunoblot analysis showing protein levels of CD133, as well as secreted MMP2 and MMP9, in different CRISPR-Cas9 melanoma cell lines and BAKP; β-Actin verified equal protein loading. Immunoblot analysis shows decreased levels of CD133 and secreted MMPs in CRISPR Cas-9 CD133 knockdown melanoma pooled clone T1 and T3, compared to BAKP and scrambled control cells. (b) Representative images of crystal violet stained invading cells after 48 hours in transwell invasion chambers; HT1080 and MCF-7 served as positive and negative controls, respectively. (c) Percent invasion of CRISPR Cas-9 CD133 knockdown cell lines (T1, T2, T3) compared to BAKP or scrambled (SC) control cells; *, **, *** represent p < 0.05, p < 0.01, or p < 0.001 respectively compared to T3 clone.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cancers