Figures for Diaz Verdugo et al., 2019

Figure Caption/Comments:

Fig. 2

Brain regions are differentially recruited during epileptic activity. a An optical section of a zebrafish larva expressing GCaMP6sin all neurons, obtained by two-photon microscopy, dorsal view (left). Individual neurons (right) in color-coded brain regions: telencephalon (dark blue), thalamus (light blue), optic tectum (green), cerebellum (yellow), and brainstem (orange). b Activity of individual neurons (ΔF/F) over time, organized by brain region. White dashed line indicates the start of 20 mM pentylenetetrazole (PTZ) perfusion. Warmer colors indicate stronger activity. cd Percentage of active neurons (>3stdbaseline) in the whole brain (c), and per brain area (d) during baseline (black), preictal (gray) and seizure periods (red). ef Average activity of the active neurons, defined by the area under the curve of the ΔF/F trace, in the whole brain (e) and per brain area (f). g Confocal image of Tg(gad1:GFP);Tg(vglut2a:dsRED) double transgenic zebrafish larva showing glutamatergic (magenta) and GABAergic (green) neurons. White bars reflect 100 µm. **p = < 0.01, *p = < 0.05, ns = not significant, Wilcoxon signed-rank test. Error bars (c-f) represent the s.e.m. of n = 8 fish, where 2012.6 ± 238.7 (mean ± SEM) neurons/fish were analyzed

Figure Data:
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