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Fig. 3

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ZDB-IMAGE-190808-10
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Figures for Marass et al., 2019
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Fig. 3

span18b as a novel vascular development gene. (A) Venn diagram illustrating the cross-analysis of the different datasets used to identify genes downstream of Npas4l. (B) IGV visualization of Npas4l occupancy at the tspan18b locus. (C) tspan18b in situ hybridization in 16 and 24 hpf npas4l+/+ and npas4l−/− embryos. (D) Maximal intensity projections of confocal z-stacks of 36 hpf Tg(fli1a:EGFP) wild-type sibling and tspan18b mutant embryos. (E) Quantification of the intersegmental vessels connected to the dorsal longitudinal anastomotic vessel in tspan18b mutants and wild-type siblings. Each dot represents the number of complete vessels in one embryo. n=24 embryos from three independent clutches. Data are mean±s.d. **P<0.01, t-test. Scale bars: 100 μm in C; 50 μm in D.

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